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KMID : 0363619970180010375
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Journal of Korean Oriental Medicine
1997 Volume.18 No. 1 p.375 ~ p.384
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Effects of Juglandis Semen extraction on oxidant-induced cell injury in lung tissues
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Lee Woo-Heon
Jung Ji-Chun
Seo Un-Kyo
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Abstract
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This study was undertaken to determine Juglandis Semen extraction (JS) has a protective effect against the cell injury caused by oxidants, t-butylhydroperoxide (t-BHP) and H_{2}O_2 in rabbit lung slices. Cell injury was estimated by measuring tissue water content and peroxidation of membrane lipids was assessed by measuring malondialdehyde (MDA), an end-product of lipid peroxidation. t-BHP significantly increased water content in lung tissues over concentrations of 2-10 mM, and such effects were prevented by 5% JS. JS exerted the beneficial effect in a dose-dependent manner. H_{2}O_2 (100 mM) also increased water content in tissues, which was almost completely prevented by 5% JS. t-BHP induced lipid peroxidation in a dose-dependent fashion in lung tissues over concentrations of 0.5-10 mM. JS significantly reduced t-BHP induced lipid peroxidation and oxidant-independent endogenous lipid peroxidation, and such effects were dose-dependent at concentration of 0.5-10%. JS prevented H_{2}O_2 (100 mM)-dependent lipid peroxidation. These results suggest that JS prevents ceil injury induced by oxidants in the lung, and such effects may be attributed to inhibition of lipid peroxidation. The precise mechanisms remains to be explored.
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KEYWORD
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Juglandis Semen, oxidant, water content, lipid peroxidation, rabbit lung slices
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